Coding

Part:BBa_K258100:Design

Designed by: Cihan Tastan   Group: iGEM09_METU-Gene   (2009-10-10)

PrtD domain of ABC transporter of Erwinia chrysanthemi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 130
    Illegal PstI site found at 713
    Illegal PstI site found at 1060
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 130
    Illegal PstI site found at 713
    Illegal PstI site found at 1060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1179
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 130
    Illegal PstI site found at 713
    Illegal PstI site found at 1060
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 130
    Illegal PstI site found at 713
    Illegal PstI site found at 1060
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene actually will be fully function with other two genes which are PrtE and PrtF

Source

E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights. Sylvie Letoffe et al. tentatively assigned the 60 kd protein to the prtD gene product (protein PrtD).

References

[1]Protease secretion by Erwinia chrysanthemi: the specific secretion functions are analogous to those of Escherichia coli c!-haemolysin Sylvie Letoffe, Philippe Delepelaire and Cecile Wandersman Unite de Gdnetique Moleculaire (URA CNRS 1149), Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France The EMBO Journal vol.9 no.5 pp. 1 375 - 1382, 1990
[2]http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=protein&dopt=GenPept&RID=D16VV617012&log%24=protalign&blast_rank=1&list_uids=581096